{"id":329,"date":"2021-01-07T00:53:57","date_gmt":"2021-01-06T19:53:57","guid":{"rendered":"http:\/\/curesteps.org\/SCI\/?p=329"},"modified":"2021-01-07T00:54:00","modified_gmt":"2021-01-06T19:54:00","slug":"chondroitinase-abc-promotes-functional-recovery-after-spinal-cord-injury-2","status":"publish","type":"post","link":"http:\/\/curesteps.org\/SCI\/chondroitinase-abc-promotes-functional-recovery-after-spinal-cord-injury-2\/","title":{"rendered":"Chondroitinase ABC promotes functional recovery after spinal cord injury"},"content":{"rendered":"\n<p><a href=\"https:\/\/www.nature.com\/articles\/416636a\">Link<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Abs1\">Abstract<\/h2>\n\n\n\n<p>The inability of axons to regenerate after a spinal cord injury in the adult mammalian central nervous system (CNS) can lead to permanent paralysis. At sites of CNS injury, a glial scar develops, containing extracellular matrix molecules including chondroitin sulphate proteoglycans (CSPGs)<sup><a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR1\">1<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR2\">2<\/a><\/sup>. CSPGs are inhibitory to axon growth&nbsp;<em>in vitro<\/em><sup><a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR3\">3<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR4\">4<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR5\">5<\/a><\/sup>, and regenerating axons stop at CSPG-rich regions&nbsp;<em>in vivo<\/em><sup><a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR6\">6<\/a><\/sup>. Removing CSPG glycosaminoglycan (GAG) chains attenuates CSPG inhibitory activity<sup><a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR7\">7<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR8\">8<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR9\">9<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/416636a#ref-CR10\">10<\/a><\/sup>. To test the functional effects of degrading chondroitin sulphate (CS)-GAG after spinal cord injury, we delivered chondroitinase ABC (ChABC) to the lesioned dorsal columns of adult rats. We show that intrathecal treatment with ChABC degraded CS-GAG at the injury site, upregulated a regeneration-associated protein in injured neurons, and promoted regeneration of both ascending sensory projections and descending corticospinal tract axons. ChABC treatment also restored post-synaptic activity below the lesion after electrical stimulation of corticospinal neurons, and promoted functional recovery of locomotor and proprioceptive behaviours. Our results demonstrate that CSPGs are important inhibitory molecules&nbsp;<em>in vivo<\/em>&nbsp;and suggest that their manipulation will be useful for treatment of human spinal injuries.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Link Abstract The inability of axons to regenerate after a spinal cord injury in the adult mammalian central nervous system (CNS) can lead to permanent paralysis. At sites of CNS injury, a glial scar develops, containing extracellular matrix molecules including chondroitin sulphate proteoglycans (CSPGs)1,2. CSPGs are inhibitory to axon growth&nbsp;in vitro3,4,5, and regenerating axons stop [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[21],"class_list":["post-329","post","type-post","status-publish","format-standard","hentry","category-scar-mitigation","tag-chondroitinase"],"_links":{"self":[{"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/posts\/329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/comments?post=329"}],"version-history":[{"count":1,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/posts\/329\/revisions"}],"predecessor-version":[{"id":330,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/posts\/329\/revisions\/330"}],"wp:attachment":[{"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/media?parent=329"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/categories?post=329"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/curesteps.org\/SCI\/wp-json\/wp\/v2\/tags?post=329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}